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Key Documents

05-1248

Sigma-Aldrich

Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306

clone CMA306, from mouse

Sinónimos:

H3K9me1, Histone H3 (mono methyl K9), H3-K9-HMTase 1, Histone H3-K9 methyltransferase 1, Lysine N-methyltransferase 1A, Position-effect variegation 3-9 homolog, Su(var)3-9 homolog 1, Suppressor of variegation 3-9 homolog 1, histone-lysine N-methyltransfe

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

CMA306, monoclonal

reactividad de especies

human

técnicas

inhibition assay: suitable (peptide)
western blot: suitable

isotipo

IgG2a

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

wet ice

modificación del objetivo postraduccional

monomethylation (Lys9)

Información sobre el gen

human ... H3C1(8350)

Descripción general

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidad

This antibody recognizes monomethylated Lys9 at and around the N-terminal of Histone H3.

Inmunógeno

Epitope: monomethylated H3 Lys9
KLH conjugated linear peptide corresponding to monomethylated Lys9 at the N-terminal region of Histone H3.

Aplicación

Dot Blot Analysis: AbSurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No. 05-1248, Anti-monomethyl H3 (Lys9) at 2ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

Peptide Inhibition: Representative lot data. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in western blot analysis of HeLa acid extracts and various other modified histone peptides.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 (Mouse Monoclonal Antibody) validated in WB, PIA to detect monomethyl Histone H3 (Lys9) also known as H3K9me1.

Calidad

Evaluated by Western Blot on recombinant Histone H3 and HeLa acid extract.
Western Blot Analysis: 1 µg/mL of this antibody detected Histone H3 in 500 ng of recombinant Histone H3 and 10 µg of HeLa acid extract.

Descripción de destino

~17 kDa

Forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2a in PBS with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable at 2-8°C for 1 year from date of receipt.

Nota de análisis

Control
Recombinant Histone H3 and HeLa acid extract.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Jarid2 (Jumonji, AT rich interactive domain 2) regulates NOTCH1 expression via histone modification in the developing heart.
Mysliwiec, MR; Carlson, CD; Tietjen, J; Hung, H; Ansari, AZ; Lee, Y
The Journal of Biological Chemistry null

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