04-1501

Anti-B7-H3 (CD276) Antibody, clone MJ8

clone MJ8, from rat

• Sinónimos: B7 homolog 3, CD276 antigen, CD276 molecule, Costimulatory molecule
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clon: MJ8, monoclonal
• application: FACS; IHC; WB
• reactividad de especies: human
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable

Propiedades

• origen biológico: rat
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: MJ8, monoclonal
• reactividad de especies: human
• reactividad de especies (predicha por homología): mouse (based on 100% sequence homology)
• técnicas: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotipo: IgG2bκ
• Nº de acceso NCBI: NP_598744.1
• Nº de acceso UniProt: Q8VE98
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CD276(80381)

Descripción

Descripción general

B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

Especificidad

This antibody recognizes the extracellular domain of B7-H3 (CD276).

Inmunógeno

Epitope: Extracellular Domain

Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.

Aplicación

Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008).

Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Calidad

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.

Descripción de destino

~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.

Forma física

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
Human placenta tissue lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable