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Key Documents

04-001

Sigma-Aldrich

Anti-JAK2 Antibody, clone 8E10.2

clone 8E10.2, Upstate®, from mouse

Sinónimos:

Janus kinase 2, Tyrosine kinase

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

8E10.2, monoclonal

reactividad de especies

mouse, human, rat

envase

antibody small pack of 25 μg

fabricante / nombre comercial

Upstate®

técnicas

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotipo

IgG1κ

Nº de acceso NCBI

Nº de acceso UniProt

Condiciones de envío

ambient

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... JAK2(3717)
mouse ... Jak2(16452)
rat ... Jak2(24514)

Descripción general

JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerizes and translocates to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

Especificidad

This antibody recognizes Jak2.

Inmunógeno

KLH-conjugated linear peptide.

Aplicación

Western Blot Analysis: 0.5-1 μg/mL of this lot was used to detect Jak2 in L6 cell lysates.

Immunohistochemistry(Paraffin):
A previous lot of this antibody was used in immunohistochemistry(paraffin) staining with eptiope retrieval: Human Cancer Array.
Anti-JAK2 Antibody, clone 8E10.2 detects level of JAK2 & has been published & validated for use in WB, IH(P).
Research Category
Inflammation & Immunology
Research Sub Category
Immunological Signaling

Calidad

Routinely evaluated by western blot on L6 cell lysate.

Descripción de destino

130 kDa

Forma física

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Nota de análisis

Control
L6 cell lysate

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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