03-903
GFP, FLAG-tagged SNRNP70 mRNA
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About This Item
Código UNSPSC:
41116158
NACRES:
NA.52
Productos recomendados
envase
1 ea of 50 μg
Nivel de calidad
fabricante / nombre comercial
Millipore
modo de crecimiento
N/A
técnicas
RIP: suitable
cell culture | mammalian: suitable
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
-65 to -96°C
Aplicación
Both mRNAs and non-coding RNAs interact with RNA-binding proteins and regulate critical cellular processes and their dysregulation contributes to multiple diseases. Thus, analyzing RNA-protein interactions are critical for understanding the mechanism of diseases and designing clinical strategies.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.
RNA-binding protein immunoprecipitation (RIP) has been used to interrogate RNA binding proteins (RBP) associated with specific RNA molecules. However, the experiments are not feasible because of the unavailability of antibodies for the particular RBP or low expression of the RBP. To overcome these issues epitope tags have been used for RIP experiments.
Características y beneficios
The GFP, FLAG-tagged SNRNP70 mRNA was developed to enable to perform control RIP experiments with RIPAb+ FLAG (Cat. No. 03-901) or RIPAb+ GFP (Cat. No. 03-902) RIP validated antibody and primer kits, and Magna RIP kit (Cat. # 17-700).
Almacenamiento y estabilidad
Store at -80ºC
Otras notas
The high amount of GFP-FLAG-tagged SNRNP70 protein is expressed immediately after transfection to cells and the well-known specific interaction of SNRNP70 and U1snRNA can be used for control RIP experiments with anti-GFP or anti-FLAG antibodies.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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