D4551

N,N-Dimethylformamide

for molecular biology, ≥99%

• Synonym(s): DMF, NSC 5356
• Linear Formula: HCON(CH₃)₂
• CAS Number: 68-12-2
• Molecular Weight: 73.09
• Beilstein: 605365
• EC Number: 200-679-5
• MDL number: MFCD00003284
• UNSPSC Code: 12352111
• PubChem Substance ID: 24893883
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 2.5 (vs air)
• vapor pressure: 2.7 mmHg ( 20 °C)
• Assay: ≥99%
• form: liquid
• autoignition temp.: 833 °F
• expl. lim.: 15.2 %
• impurities: ≤0.5% water; water
• refractive index: n20/D 1.430 (lit.)
• bp: 153 °C (lit.)
• mp: −61 °C (lit.)
• density: 0.944 g/mL (lit.)
• suitability: suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications.
• storage temp.: room temp
• SMILES string: [H]C(=O)N(C)C
• InChI: 1S/C3H7NO/c1-4(2)3-5/h3H,1-2H3
• InChI key: ZMXDDKWLCZADIW-UHFFFAOYSA-N

Description

General description

N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications.

Application

N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme.

Solvent for many hydrophobic organic compounds.

Safety Information

• Pictograms: GHS02,GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H226,H312 + H332,H319,H360D
• Precautionary Statements: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2
• Flash Point(F): 135.5 °F - closed cup
• Flash Point(C): 57.5 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves