CHO-K1 Cell Line from hamster

from hamster ovary, 85051005

• Synonym(s): CHO cell clone K1 Cells, CHOK1 Cells
• UNSPSC Code: 41106514

Properties

• biological source: hamster ovary
• growth mode: Adherent
• karyotype: Hypodiploid, modal no. 20
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Hamster Chinese ovary

Cell Line Description

A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.

Application

CHO-K1 cell line from hamster has been used to study the effect of plasmid pCM01 induction on the association of bacteria with CHO-K1 cells by the gentamicin protection assay. It has also been cultured with or without lactate to measure time-series for cell proliferation.

Nutritional and gene expression studies

Culture Medium

Ham′s F12+ 2mM Glutamine+ 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25%Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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