MABT418

Anti-GPVI Antibody, clone 8A2, Detection Antibody

clone 8A2, from rat

• Synonym(s): Platelet glycoprotein VI, GPVI, Glycoprotein 6
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8A2, monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001242946
• UniProt accession no.: Q9HCN6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GP6(51206)

Description

General description

Glycoprotein VI (GPVI), or glycoprotein 6 (GP6), is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. GPVI is a collagen receptor involved in collagen-induced platelet adhesion and activation, and may contribute to arterial and venous thrombus formation. GPVI is a membrane glycoprotein that plays a crucial role in platelet procoagulant activity and subsequent thrombin and fibrin formation. GPVI is expressed in megakaryocytes and platelets. The signaling pathway of GPVI involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. It has been shown that bleeding disorders such as Bleeding Disorder Platelet-Type 11 (BDPLT11), a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen, is caused by defects in GPVI.

Immunogen

Recombinant protein corresponding to human GPVI.

Application

Anti-GPVI Antibody, clone 8A2, Detection Antibody is an antibody against GPVI for use in western blotting & ELISA.

ELISA Assay: A representative lot from an independent laboratory detected recombinant GPVI in a bead-based sandwich immunoassay (Bigalke, B., et al. (2011). Clin Chem. 57(6):898-904.). This antibody should be used in conjunction with Cat. No. MABT406, Anti-GPVI, clone 8E9, Capture Antibody, as an ELISA detection and capture antibody pair.

Quality

Evaluated by Western Blotting in recombinant human GPVI glycoprotein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human GPVI glycoprotein.

Target description

~50 kDa observed. The calculated molecular weight of this protein is 37 kDa However, a recombinant protein was observed at 50-55 kDa in Western Blotting.

Linkage

Replaces: MABT473

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable