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Key Documents

MAB371

Sigma-Aldrich

Anti-Cranin Antibody, aa572-604 of human sequence, clone 6C1

ascites fluid, clone 6C1, Chemicon®

Synonym(s):

alpha-dystroglycan

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6C1, monoclonal

species reactivity

mouse, sheep, rat, human, chicken, Xenopus

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgM

UniProt accession no.

Q9TS66

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... DAG1(1605)

Specificity

Cranin (dystroglycan), extracellular site, near the C-terminus of the alpha-subunit. The expression and localization of cranin varies considerably according to the cells physiologic state and differentiaion state. MAB371 recognizes the same peptide region as MAB373 but a different epitope.

Immunogen

Epitope: aa572-604 of human sequence
Synthetic peptide corresponding to a.a. 572-604 of human cranin.

Application

Immunohistochemistry on tissue fixed briefly with 0.5% glutaraldehyde, 2% PLP fixation, methanol or acetone. Prolonged fixation must be avoided. Traditional formalin is not recommended. Suggested working dilution 1:1-1:50.

Immunocytochemistry on neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.

ELISA

Not suggested for use in Western blot.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure

Cell Structure
Research Sub Category
Cytoskeleton

ECM Proteins
This Anti-Cranin Antibody, aa572-604 of human sequence, clone 6C1 is validated for use in ELISA, IC, IH for the detection of Cranin.

Physical form

Ascites Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Purificaiton of Cranin, a laminin binding membrane protein
Smalheiser, NR and Kim, E.
The Journal of Biological Chemistry, 270, 15425-15433 (1995)
B J Collins et al.
Endocrinology, 142(7), 3152-3162 (2001-06-21)
Dystroglycan is a high affinity laminin-binding glycoprotein originally described as a member of the dystrophin-associated glycoprotein complex in muscle. We have demonstrated the presence of dystroglycan in the thyroid using immunocytochemistry, immunoblots, ligand binding assays, and relative quantitative RT-PCR. In
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to

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