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Key Documents

M1533

Sigma-Aldrich

N,N′-Methylenebisacrylamide solution

suitable for electrophoresis, 2% in H2O

Synonym(s):

BIS

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About This Item

Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
Molecular Weight:
154.17
Beilstein:
1706297
MDL number:
UNSPSC Code:
41105319
PubChem Substance ID:
NACRES:
NB.22

sterility

0.2 μm filtered

Quality Level

form

liquid

concentration

2% in H2O

technique(s)

electrophoresis: suitable

storage temp.

2-8°C

SMILES string

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)

InChI key

ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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General description

N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.

Application

N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication. It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.
To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Preparation Note

Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B - Muta. 1B - STOT RE 2 Oral

Target Organs

Peripheral nervous system

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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A J Aho et al.
British journal of anaesthesia, 115(2), 258-266 (2015-07-04)
Concomitantly recorded Bispectral Index® (BIS) and Entropy™ values sometimes show discordant trends during general anaesthesia. Previously, no attempt had been made to discover which EEG characteristics cause discrepancies between BIS and Entropy. We compared BIS and Entropy values, and analysed
Bjørn Bjorvatn et al.
Sleep & breathing = Schlaf & Atmung, 19(4), 1387-1393 (2015-04-10)
The objective of the present study was to investigate the prevalence of insomnia and excessive sleepiness in relation to the presence and severity of obstructive sleep apnea (OSA). The sample consisted of patients referred to a university hospital on suspicion
Jean Guglielminotti et al.
Anesthesiology, 122(5), 985-993 (2015-03-03)
Individual assessment of the amplitude of a physiologic reflex evoked by a standardized noxious test (SNT) before surgical stimulation has been suggested to predict movement upon the forthcoming surgical stimulation. This study aimed to compare the ability of pupillary dilatation
Yan Lu et al.
ACS nano, 4(12), 7078-7086 (2010-11-19)
Here, we demonstrate that bimetallic Au-Pt nanorods (NRs) can be grown in situ into thermosensitive core-shell microgel particles by a novel two-step approach. In the first step, Au NRs with an average width of 6.6 ± 0.3 nm and length
David R Kryscio et al.
Analytica chimica acta, 718, 109-115 (2012-02-07)
Molecularly imprinted polymers are synthetic antibody mimics formed by the crosslinking of organic or inorganic polymers in the presence of an analyte which yields recognitive polymer networks with specific binding pockets for that biomolecule. Surface imprinted polymers were synthesized via

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