L0543

LB Agar Kanamycin-50, Plates

pre-poured LB agar plates with 50 ug/ml kanamycin

• Synonym(s): kanamycin agar plates
• UNSPSC Code: 41121800
• sterility: sterile; autoclaved
• suitability: selective for Escherichia coli; selective for coliforms

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: sterile; autoclaved
• form: plates (10 cm); plates (10cm, 20-25ml each)
• capacity: 20-25 mL
• storage temp.: 2-8°C
• suitability: selective for Escherichia coli; selective for coliforms

Description

General description

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for selective cultivation of E. coli strains that contain plasmids conferring kanamycin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins. LB Agar Kanamycin-50, Plates has been used in the screening of Agrobacterium tumefaciens and E.coli JM83 strain with pGD103 plasmid.

Features and Benefits

Miller′s LB Agar plates provide:

• Convenient, ready-to-use 10cm format
• Standard formulation plus antibiotic

Components

15g/L Agar
10g/L Tryptone
10g/L NaCl
5g/L Yeast Extract
50μg/ml kanamycin

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable