L3022
LB Broth (Lennox)
Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
Synonym(s):
Lennox broth, Lennox broth , Luria Bertani Broth
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.85
grade
for molecular biology
Quality Level
sterility
non-sterile
form
powder
composition
NaCl, 5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
Related Categories
General description
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids and carbohydrates in a low-salt formulation.
Application
Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
It was used for resuspension of T. pyriformis cell fractions in a study of Trimastix fractionation of cellular extracts.
It was used for resuspension of T. pyriformis cell fractions in a study of Trimastix fractionation of cellular extracts.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
Features and Benefits
Lennox LB powder provides:
- Convenient small package to eliminate weighing
- Easy scale-up using larger package sizes
- A budget-friendly alternative to liquid
- Standard formulation
Preparation Note
1. Suspend 20 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.
2. Autoclave for 15 minutes at 121 °C.
To prepare Lennox L Broth: Add 1 g glucose and proceed with preparation instructions as above.
To prepare the medium of Enquist and Sternberg: Aseptically add 10 ml of sterile 1 M magnesium sulfate after autoclaving.
Legal Information
Zeocin is a registered trademark of Cayla Sarl
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Protocols
General protocols for growth of competent cells in microbial medium.
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