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89916

Millipore

Modified Lauryl Sulfate Tryptose Broth acc.to ISO/TS 22964

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Lauryl Sulfate Tryptose Broth, modified, mLST

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

Quality Level

200

Agency

according to GB 4789.40-2016

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium phosphate, 2.75 g/L
enzymatic digest of plants and animal tissue, 20 g/L
lactose, 5 g/L
lauryl sulfate, 0.1 g/L
monopotassium phosphate, 2.75 g/L
sodium chloride, 34 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
sample preparation
water monitoring
microbiology

suitability

selective for Cronobacter spp.

Application

Modified Lauryl Sulfate Tryptose Broth is recommended by ISO Committee as selective enrichment medium for Enterobacter sakazakii from milk and milk products.

Preparation Note

Dissolve 64.6 grams in 1000 ml distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add rehydrated contents of 1 vial of Vancomycin Supplement (Cat. No. 75423). Mix well and distribute in tubes as desired so as to obtain final concentration of 10μg of vancomycin per ml of the medium.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG9937
BCCF1896
BCCD8156
BCBZ3908
BCBT7445

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O Guillaume-Gentil et al.
Journal of food protection, 68(1), 64-69 (2005-02-05)
A method was developed to detect and identify Enterobacter sakazakii in environmental samples. The method is based on selective enrichment at 45+/-0.5 degrees C in lauryl sulfate tryptose broth supplemented with 0.5 M NaCl and 10 mg/liter vancomycin (mLST) for

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