Direkt zum Inhalt
Merck
  • Isolation and characterization of a trypsin inhibitor from the seeds of kohlrabi (Brassica napus var. rapifera) belonging to the napin family of storage proteins.

Isolation and characterization of a trypsin inhibitor from the seeds of kohlrabi (Brassica napus var. rapifera) belonging to the napin family of storage proteins.

Carlsberg research communications (1989-01-01)
I B Svendsen, D Nicolova, I Goshev, N Genov
ZUSAMMENFASSUNG

A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined by amino acid sequence analysis which also showed the inhibitor to be polymorphous. The inhibitor has been further characterized by means of molecular weight determination using SDS gel-electrophoresis and by amino acid analysis, fluorimetry as well as circular dichroism. A simplified method for purification of napins is given.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), lyophilized powder
Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin-Inhibitor aus Hühnereiweiss, Type II-O, Partially purified ovomucoid, containing ovoinhibitor
Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), lyophilized powder
Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), solution, sterile-filtered, suitable for, suitable for cell culture
Sigma-Aldrich
Trypsin-Inhibitor aus Hühnereiweiss, Type III-O (free of ovoinhibitor)
Sigma-Aldrich
Trypsin-Inhibitor aus Phaseolus limensis (lima bean), Type II-L, crude powder
Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), lyophilized powder, ~10000 U/mg
Sigma-Aldrich
Trypsin Inhibitor, Defined (1X) Solution, Animal component free, BioReagent, suitable for cell culture
Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), For use as a marker in SDS-PAGE, BioReagent
Sigma-Aldrich
Trypsin-Inhibitor aus Glycine max (soybean), Isoelectric focusing marker, pI 4.6