Fate of desmosomal proteins in apoptotic epidermal cells.

Fate of desmosomal proteins in apoptotic epidermal cells.

Methods in molecular biology (Clifton, N.J.) (2004-10-27)

Jörg Weiske, Otmar Huber

ZUSAMMENFASSUNG

In epidermal cells desmosomes represent major sites of basolateral cell-cell contacts that play an important role for epidermal homeostasis. Excess or damaged cells are often removed by apoptosis. To release apoptotic cells from the tissue, intercellular contacts have to be broken. Here detailed protocols to analyze desmosomal proteins in apoptotic cells are described.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

G2500

Sigma-Aldrich

Gelatine aus Schweinehaut, gel strength 300, Type A

S5921

Sigma-Aldrich

Staurosporin aus Streptomyces sp., for molecular biology, ≥95% (HPLC)

C9911

Sigma-Aldrich

(S)-(+)-Camptothecin, ≥90% (HPLC), powder

G6767

Sigma-Aldrich

Ziegenserum aus kontrollierter Spenderherde, USA origin, sterile-filtered, suitable for cell culture

264155

Sigma-Aldrich

Caspase-3-Inhibitor II, The Caspase-3 Inhibitor II, also referenced under CAS 210344-95-9, controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

444250

Sigma-Aldrich

MMP-Inhibitor I, The MMP Inhibitor I controls the biological activity of MMP. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.