G005887

LC/MS (TOF) Analysis of DL-α-Hydroxyglutaric Acid Enantiomers on Astec^® CHIROBIOTIC^® R, Fast Separation

application for HPLC

Eigenschaften

• Methode(n): HPLC: suitable
• Testparameter: column: Astec CHIROBIOTIC^® R, 10 cm x 4.6 mm I.D., 5μm particles (13022AST); mobile phase: 5:95, (water:methanol) with 0.3% acetic acid and 0.1% ammonium hydroxide; flow rate: 1 mL/min; pressure: 1700 psi (117 bar); column temp.: 25 °C; detector: MS, ESI(-) TOF, SIM, m/z 147.03, split 100:1; injection: 10 μL; sample: DL-a-Hydroxyglutaric acid, 1 mg/mL in 50:50, water:methanol
• Eignung: application for HPLC
• Anwendung(en): clinical

Beschreibung

Hinweis zur Analyse

Chiral 2-hydroxyglutarates (2-OHG) are important molecular signatures of both healthy and diseased biological cells, their specific biochemical pathways and inborn errors of metabolism. The chiral differentiation and quantification of D-2-OHG and L-2-OHG is key for characterizing neurometabolic disorders like the 2-hydroxyglutaric acidurias that cause neurological impairment early in life. In this application, an Astec^® CHIROBIOTIC^® R column run under polar ionic mobile phase conditions achieved a rapid separation of the D and L enantiomers of 2-OHG under conditions amenable for sensitive LC-MS/MS detection.

Rechtliche Hinweise

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyt

DL-α-Hydroxyglutaric acid disodium salt, ≥95.0% (GC)