823425

TOYOPEARL^® AF-rProtein A HC-650F Bulk Media

phase rProtein A, bottle of 10 mL, 45 μm particle size

• Synonym(e): TOYOPEARL^® AF-rProtein A HC-650F Bulk Media
• UNSPSC-Code: 41106500

Eigenschaften

• Beschreibung: Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time)); Clean in Place (CIP) (0.1 M NaOH); Phase filter (Protein A)
• Produktlinie: TOYOPEARL^®
• Form: slurry
• Verpackung: bottle of 10 mL
• Hersteller/Markenname: Tosoh 23425
• Parameter: 3 bar max. pressure
• Methode(n): LPLC: suitable
• Matrix: hydroxylated methacrylic polymer particle platform
• Aktive Matrixgruppe: rProtein A phase
• Partikelgröße: 45 μm
• Porengröße: 1000 Å
• pH-Wert: 2-13
• Kapazität: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• Trenntechnik: affinity
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

Spezifikationen

Clean in place with 0.1 M NaOH.

Physikalische Form

72% (v/v) slurry in 20% (v/v) ethanol

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Rechtliche Hinweise

Toyopearl is a registered trademark of Tosoh Corporation

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P210 - P233 - P240 - P241 - P242 - P243
• Gefahreneinstufungen: Flam. Liq. 3
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 3
• Flammpunkt (°F): 104.0 °F - closed cup
• Flammpunkt (°C): 40 °C - closed cup