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TOYOPEARL® HW-40F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm

Synonym(e):

TOYOPEARL® HW-40F

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About This Item

UNSPSC-Code:
41115700

Beschreibung

HW-40F

Produktlinie

TOYOPEARL®

Form

slurry

Verpackung

bottle of 500 mL

Parameter

3 bar max. pressure

Methode(n)

HPLC: suitable

Matrix

hydroxylated methacrylic polymer

Aktive Matrixgruppe

hydroxylated methacrylate phase

Partikelgröße

30-60 μm

Porengröße

100-7000 Da MW range

Betriebs-pH-Wert

2-13

Trenntechnik

size exclusion (SEC)

Allgemeine Beschreibung

Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

Anwendung

Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Spezifikationen

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physikalische Form

Shipped in 20% (v/v) ethanol.

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Rechtliche Hinweise

Toyopearl is a registered trademark of Tosoh Corporation

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Gefahreneinstufungen

Flam. Liq. 3

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 1

Flammpunkt (°F)

103.1 °F

Flammpunkt (°C)

39.5 °C

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
107H0370
097H0647

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Die Dokumentenbibliothek aufrufen

Francesca Magnani et al.
Nature protocols, 11(8), 1554-1571 (2016-07-29)
The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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