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Merck

ZMS1056

Sigma-Aldrich

Anti-Nuclei Antibody, clone 3E1.3 ZooMAb® Mouse Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Rekombinant

expressed in HEK 293 cells

Konjugat

unconjugated

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

3E1.3, recombinant monoclonal

Produktlinie

ZooMAb® learn more

Form

lyophilized

Speziesreaktivität

human

Verpackung

antibody small pack of 25 μL

Grünere Alternativprodukt-Eigenschaften

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

Erweiterte Validierung

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

Methode(n)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

Isotyp

IgG1

Epitopsequenz

Unknown

UniProt-Hinterlegungsnummer

Grünere Alternativprodukt-Kategorie

Versandbedingung

ambient

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spezifität

Clone 3E1.3 is a ZooMAb® mouse recombinant monoclonal antibody that specifically detects human cell nuclei.

Immunogen

Purified nuclei from human cells.

Anwendung

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Nuclei in human cerebral cortex tissue sections.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nuclei in human kidney tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nuclei in HeLa, A431 and HepG2 cells.

Flow Cytometry Analysis: 1 μg from a representative lot detected Nuclei in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Nuclei, clone 3E1.3 ZooMAb®, Cat. No. ZMS1056, is a recombinant Mouse monoclonal antibody that detects human nuclei and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Flow Cytometry.

Zielbeschreibung

The nucleus is a membrane-enclosed organelle found in eukaryotic cells that contains the genetic information of the cell. In the nucleus the integrity of genes is maintained. Nuclear marker antibodies are useful tools to study the morphology and dynamics of the nucleus and provide a means for monitoring changes in nucleus during different phases of the cell cycle. Clone 3E1.3 is a mouse monoclonal antibody that serves as an excellent marker for nuclei in human cells, including neurons and embryonic stem cells. However, it does not react with rat or mouse cell nuclei. It gives a diffuse nuclear staining pattern. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physikalische Form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstituierung

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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