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W4128

Sigma-Aldrich

Williams‘ Medium E

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

Williams’ E medium

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75

product name

Williams‘ Medium E, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sterilität

sterile-filtered

Form

liquid

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Komponenten

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

Versandbedingung

ambient

Lagertemp.

2-8°C

Allgemeine Beschreibung

Williams′ Medium E has been shown to support the growth in long-term culture of adult liver epithelial cells.

Anwendung

Williams′ Medium E has been used to culture primary human hepatocytes and to maintain hepatocellular carcinoma (JHH-6) cell lines.

Rekonstituierung

Supplement with 0.292 g/L L-glutamine.

Ergänzung

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

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Hannah L Paish et al.
Hepatology (Baltimore, Md.), 70(4), 1377-1391 (2019-04-10)
Precision cut liver slices (PCLSs) retain the structure and cellular composition of the native liver and represent an improved system to study liver fibrosis compared to two-dimensional mono- or co-cultures. The aim of this study was to develop a bioreactor
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Liver research, 5(1), 16-20 (2021-08-06)
Mitophagy is a lysosomal degradation pathway that selectively removes damaged, aged and dysfunctional mitochondria. Recent advances in understanding mitophagy highlight its importance in various physiological and pathological conditions including liver diseases. However, reliable quantitative assays to monitor mitophagy in cultured
Wen-Xing Ding et al.
Methods in enzymology, 453, 397-416 (2009-02-17)
Mammalian autophagy has been well characterized in the liver and in hepatocytes. Autophagy plays important roles in the normal physiology of the liver and in the pathogenesis of several liver diseases. This chapter will discuss the commonly used methods for
Dongxin Zhao et al.
PloS one, 4(7), e6468-e6468 (2009-08-04)
The derivation of hepatic progenitor cells from human embryonic stem (hES) cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here
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