Direkt zum Inhalt
Merck
Alle Fotos(1)

Dokumente

10108014001

Roche

Papain

from Carica papaya

Synonym(e):

cystein protease, papain

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
Alle Fotos(1)

About This Item

EC-Nummer:
3.4.22.2 (BRENDA, IUBMB)
UNSPSC-Code:
12352204

Biologische Quelle

papaya

Qualitätsniveau

100

Form

suspension

Spezifische Aktivität

30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)

Mol-Gew.

23 kDa

Verpackung

pkg of 10 mL (100 mg)

Hersteller/Markenname

Roche

Konzentration

10-100 mg/mL

Optimaler pH-Wert

6.0-7.0

Versandbedingung

wet ice

Verwandte Kategorien

Allgemeine Beschreibung

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Anwendung

Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Biochem./physiol. Wirkung

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Leistungsmerkmale und Vorteile

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Physikalische Form

Suspension, crystalline, nonsterile

Angaben zur Herstellung

Working concentration: 0.05 to 0.5 mg/ml

Lagerung und Haltbarkeit

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

John S. White and Dorothy C. White
Source Book of Enzymes null
Spatiotemporal ablation of CXCR2 on oligodendrocyte lineage cells: Role in myelin repair
Liu L, et al.
Neurology? neuroimmunology & neuroinflammation, 2(6), e174-e174 (2015)
Bortezomib and metformin opposingly regulate the expression of hypoxia-inducible factor alpha and the consequent development of chemotherapy-induced painful peripheral neuropathy
Ludman T and Melemedjian O K
Molecular Pain, 15, 1744806919850043-1744806919850043 (2019)
LIM Kinase, a Newly Identified Regulator of Presynaptic Remodeling by Rod Photoreceptors After Injury.
Wang W and Townes-Anderson E
Investigative Ophthalmology & Visual Science, 56(13), 7847-7858 (2015)
The structure of papain
Advances in Protein Chemistry, 25, 79-115 (1971)
Alle zugehörigen Dokumente anzeigen

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

Setzen Sie sich mit dem technischen Dienst in Verbindung.