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Merck

T3413

Sigma-Aldrich

Monoclonal Anti-Tenascin antibody produced in rat

clone MTn-12, ascites fluid

Synonym(e):

Anti-Tenascin-N

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rat

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

MTn-12, monoclonal

Enthält

15 mM sodium azide

Speziesreaktivität

mouse

Methode(n)

immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:200 using unfixed, frozen tissue sections of mouse intestine
western blot: suitable

Isotyp

IgG1

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

mouse ... Tnn(329278)

Allgemeine Beschreibung

Monoclonal Anti-Mouse Tenascin (rat IgG1 isotype) is derived from the MTn-12 hybridoma1 produced by the fusion of rat myeloma cells and splenocytes from a Lou rat immunized with partially purified mouse tenascin. Human tenascin has three subunits of 190, 200 and 220 kDa. Tenascin has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer, depending on species, the molecular weights of the subunits range from 190 to 320 kDa.
Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).
The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.

Spezifität

The antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells.

Immunogen

partially purified mouse tenascin.

Anwendung

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Monoclonal Anti-Mouse Tenascin antibody may be used for the localization of tenascin and to study of the role of tenascin in epithelial-mesenchymal interactions using various immunochemical assays including ELISA, immunoblot, dot blot and immunohistology.
Monoclonal Anti-Tenascin antibody produced in rat has been used in:
  • Enzyme linked immunosorbent assay (ELISA)
  • Dot blot.
  • Immunoblotting
  • Fluorescence microscopy and immunostaining
  • Immunofluorescence
  • Immunohistochemistry

Biochem./physiol. Wirkung

Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence motif which may function in cell adhesion and may be recognized by integrin receptor.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Developmental changes in patterns of expression of tenascin-C variants in the human cornea
Maseruka H, et al.
Investigative Ophthalmology & Visual Science, 41(13), 4101-4107 (2000)
Tumor-derived Tenascin-C Promotes the Epithelial-Mesenchymal Transition in Colorectal Cancer Cells
Takahashi Y, et al.
Anticancer Research, 33(5), 1927-1934 (2013)
Clinical impact and functional aspects of tenascin-C expression during glioma progression
Herold-Mende C, et al.
International Journal of Cancer. Journal International Du Cancer, 98(3), 362-369 (2002)
Site-specific HNK-1 epitope on alternatively spliced fibronectin type-III repeats in tenascin-C promotes neurite outgrowth of hippocampal neurons through contactin-1
Nakamura A, et al.
Testing, 14(1), e0210193-e0210193 (2019)
Tumor targeting by an aptamer
Hicke BJ, et al.
Journal of Nuclear Medicine, 47(4), 668-678 (2006)

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