SRP3031

Endostatin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(e): COL18A1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Form: lyophilized
• Mol-Gew.: 20.2 kDa
• Verpackung: pkg of 100 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.1 EU/μg endotoxin, tested
• Farbe: white to off-white
• UniProt-Hinterlegungsnummer: P39060
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... COL18A1(80781)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Endostatin is an endogenous glycoprotein derived from the C-terminal portion of the α1 chain of type XVIII collagen. It is encoded by the gene mapped to human chromosome 21q22.3. Recombinant human Endostatin protein consists of 184 amino acid residues. It is expressed in various organs with the highest levels in liver, lung, and kidney.

Biochem./physiol. Wirkung

Endostatin is a potent inhibitor of angiogenesis. It exerts its angiostatic effect through multiple pathways related to endothelial cell migration and proliferation. Endostatin inhibits and suppresses the actions of matrix metalloproteinases (MMPs), FAK (Focal adhesion kinase)/Ras/p38-MAPK (mitogen-activated protein kinase)/ERK (extracellular signal-regulated kinase) signaling cascade, HIF-1α(hypoxia-inducible factor 1 alpha)/VEGF-A (vascular endothelial growth factor)thereby inhibiting angiogenesis by preventing the migration and invasion of endothelial cells. Endostatin also blocks the apoptosis of cells and the G1 to S phase transition in the cell cycle. Mutations in this gene lead to Knobloch syndrome (KS), a rare autosomal recessive disorder.

Sequenz

MHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM TASK

Physikalische Form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable