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Key Documents

SRP2030

Sigma-Aldrich

Sp1 GC-box binding protein human

recombinant, expressed in insect cells, ≥75% (SDS-PAGE)

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.26

Biologische Quelle

human

Rekombinant

expressed in insect cells

Assay

≥75% (SDS-PAGE)

Form

frozen liquid

Mol-Gew.

~81.6 kDa

Verpackung

pkg of 5 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Konzentration

200 μg/mL

Methode(n)

electrophoretic mobility shift assay: suitable

Farbe

clear colorless

NCBI-Hinterlegungsnummer

NM_138473

UniProt-Hinterlegungsnummer

P08047

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... SP1(6667)

Anwendung

Sp1 transcription factor has been used as a standard in electrophoretic mobility shift assay.

Biochem./physiol. Wirkung

Sp1 was first detected in HeLa cells on the basis of its ability to activate the SV40 early promoter transcription. Subsequently it was shown to recognize and bind selectively to a GC-rich consensus sequence (GC-box: GGGCGG or CACCC) that presents in the promoter of several important cellular genes, including SV40 early, HIV-1, PDGF-B etc. Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has revealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well-characterized motif found in several other transcription factors. In addition to transcription, Sp1 function has been linked to cell growth, cancer, Huntington disease and other disorders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation.

Physikalische Form

Clear and colorless frozen liquid solution

Angaben zur Herstellung

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

M R Briggs et al.
Science (New York, N.Y.), 234(4772), 47-52 (1986-10-03)
The biochemical analysis of cellular trans-activators involved in promoter recognition provides an important step toward understanding the mechanisms of gene expression in animal cells. The promoter selective transcription factor, Sp1, has been purified from human cells to more than 95
W S Dynan et al.
Cell, 35(1), 79-87 (1983-11-01)
Fractionation of HeLa cell extracts reveals the presence of a promoter-specific transcription factor, Sp 1, which activates a class of promoters that includes the SV40 early promoter but not several others that have been tested. We analyzed SV40 early-promoter deletion
Alexandra Jaeger et al.
International journal of biological sciences, 11(9), 1006-1015 (2015-07-30)
The beta-2 adrenergic receptor (beta-2 AR) modulates metabolic processes in skeletal muscle, liver, and adipose tissue in response to catecholamine stimulation. We showed previously that expression of the porcine beta-2 AR gene (ADRB2) is affected by cis-regulatory polymorphisms. These are

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