SRP0417

BirA

recombinant, expressed in E. coli, ≥65% (SDS-PAGE)

• Synonym(e): BirA Biotin Ligase
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Rekombinant: expressed in E. coli
• Assay: ≥65% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 37 kDa
• Verpackung: pkg of 100 μg
• NCBI-Hinterlegungsnummer: AP012306
• Versandbedingung: dry ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Biotin ligase BirA (GenBank Accession No. AP012306 (3602298-3603260)) amino acids 2 – 321 (end) with His-FLAG-tag, MW = 37.1 kDa, expressed in an Escherichia coli cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem./physiol. Wirkung

Biotin ligase BirA controls biotin synthesis in Escherichia coli. It sends biotin to metabolism. As a homodimer, it negatively controls the biotin synthetic operon. BirA also catalyzes post-translational biotinylation, by associating with biotin acceptor protein of acetyl-coenzyme A carboxylase. It can biotinylate human histones.

Physikalische Form

Formulated in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 150 mM imidazole, 3 mM DTT, and 5% Glycerol.

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H360D
• P-Sätze: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Gefahreneinstufungen: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklassenschlüssel: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable