SRP0179

Lysyl Oxidase-like 2 (LOXL2) FLAG-tag (Sf9-derived) human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

• Synonym(e): LOR2, LOXL2, WS9-14
• UNSPSC-Code: 12352204
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥65% (SDS-PAGE)
• Form: aqueous solution
• Verpackung: pkg of 10 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_002318
• UniProt-Hinterlegungsnummer: Q9Y4K0
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... LOXL2(4017)

Beschreibung

Allgemeine Beschreibung

The gene LOXL2 (lysyl oxidase like 2) is mapped to human chromosome 8p21.3. It is widely expressed and belongs to the LOX family of proteins. The protein has a conserved C-terminus region with catalytic function and a divergent N-terminal part.
Human LOXL2 (GenBank Accession No. NM_002318), amino acids 500-end, MW = 32kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem./physiol. Wirkung

LOXL2 (lysyl oxidase like 2) is an amino oxidase which is dependent on copper and quinone. LOXL2 participates in various events, such as gene transcription, cell migration, adhesion, angiogenesis and differentiation. It is responsible for connecting collagen and/or elastin in the extracellular matrix. It deaminates trimethylated lysine 4 in histone H3, causing transcriptional repression. LOXL2 also oxidises methylated TAF10 (TATA-box binding protein associated factor 10), suppressing some TFIID (transcription factor IID)-associated genes. The LOXL2 gene is upregulated in various cancers, such as gastric cancer, breast cancer and squamous cell carcinomas. The gene is associated with cancer cell invasion, lymph node metastasis and poor overall patient survival.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable