SRP0125
LSD1 substrate (Di-methylated K4_H3)
≥90% (HPLC), aqueous solution
Synonym(e):
HIST1H3E, Histone H3.1
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About This Item
UNSPSC-Code:
12352204
NACRES:
NA.32
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Produktbezeichnung
LSD1 substrate (Di-methylated K4_H3), ≥90% (HPLC)
Biologische Quelle
human
Assay
≥90% (HPLC)
Form
aqueous solution
Mol-Gew.
2283 Da
Verpackung
pkg of 500UL
Konzentration
0.55 mg/mL
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Lagertemp.
−70°C
Angaben zum Gen
human ... HIST1H3E(8353)
Allgemeine Beschreibung
Lysine-specific demethylase 1 (LSD1) acts on mono- and dimethylated histones and demethylates them. It has a role in embryogenesis, cell proliferation, spermatogenesis, adipogenesis and chromosomal segregation. Histone H3 peptide methylated at Lysine 4 is a substrate for LSD1. Histone cluster 1 H3 family member E (HIST1H3E) is a variant of histone 3 and is also called as the replicative histone. It is mainly expressed in the S-phase of the cell cycle. The gene encoding this protein is localized on human chromosome 6p22.
Anwendung
Study enzyme kinetics and screen small molecular inhibitors of LSD1 for drug discovery and HTS applications
Biochem./physiol. Wirkung
Histones have an important role in the organization and modification of chromatin. Histone cluster 1 H3 family member E (HIST1H3E) has a role in DNA synthesis during DNA replication and might also be involved in DNA repair. It is deposited at the chromatin at the time of DNA replication-associated chromatin assembly. During herpes simplex virus 1 (HSV-1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.
Physikalische Form
Supplied as 500 uL of water solution at 200 uM concetration.
Angaben zur Herstellung
Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles.
Lagerklassenschlüssel
10 - Combustible liquids
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Yingwei Chen et al.
Critical reviews in eukaryotic gene expression, 22(1), 53-59 (2012-02-22)
Lysine-specific demethylase 1 (LSD1), the first identified histone demethylase, was belonged to the superfamily of the flavin adenine dinucleotide (FAD)-dependent amine oxidases. LSD1 specifically demethylates mono- or dimethylated dimethylated histone H3 lysine4 (H3K4) and H3 lysine 9 (H3K9) via a
Kristen L Conn et al.
PLoS pathogens, 9(10), e1003695-e1003695 (2013-10-17)
During lytic infections, HSV-1 genomes are assembled into unstable nucleosomes. The histones required for HSV-1 chromatin assembly, however, are in the cellular chromatin. We have shown that linker (H1) and core (H2B and H4) histones are mobilized during HSV-1 infection
Kami Ahmad et al.
Molecular cell, 9(6), 1191-1200 (2002-06-28)
Two very similar H3 histones-differing at only four amino acid positions-are produced in Drosophila cells. Here we describe a mechanism of chromatin regulation whereby the variant H3.3 is deposited at particular loci, including active rDNA arrays. While the major H3
Thomas H A Ederveen et al.
Biochimica et biophysica acta, 1809(10), 577-586 (2011-07-26)
Histones are highly basic, relatively small proteins that complex with DNA to form higher order structures that underlie chromosome topology. Of the four core histones H2A, H2B, H3 and H4, it is H3 that is most heavily modified at the
Eric I Campos et al.
Nature structural & molecular biology, 17(11), 1343-1351 (2010-10-19)
The mechanism by which newly synthesized histones are imported into the nucleus and deposited onto replicating chromatin alongside segregating nucleosomal counterparts is poorly understood, yet this program is expected to bear on the putative epigenetic nature of histone post-translational modifications.
Artikel
Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.
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