SRP0122

LSD1 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• Synonym(e): Amine oxidase (flavin containing) domain 2 (AOF2), BRAF35-HDAC complex protein BHC110, KIAA0601, Lysine (K)-specific demethylase 1 (LSD1), Lysine-specific histone demethylase 1A (KDM1A)
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥70% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 103 kDa
• Verpackung: pkg of 50 μg
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_015013
• UniProt-Hinterlegungsnummer: O60341
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... KDM1A(23028)

Beschreibung

Allgemeine Beschreibung

Human LSD1, AOF2, amine oxidase, flavin containing, domain 2, GenBank Accession No. NM_015013, amino acids 158-end with N-terminal ST tag, MW = 103 kDa, expressed in E. coli expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable