SRP0115

Sirtuin 1 human

recombinant, expressed in E. coli, ≥60% (SDS-PAGE)

• Synonym(e): SIR2, SIRT1, Sir2A, sir2-like 1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in E. coli
• Assay: ≥60% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 87.2 kDa
• Verpackung: pkg of 100 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• NCBI-Hinterlegungsnummer: NM_012238
• UniProt-Hinterlegungsnummer: Q96EB6
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... SIRT1(23411)

Beschreibung

Allgemeine Beschreibung

Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Einheitendefinition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable