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Merck

SAB5600096

Sigma-Aldrich

Anti-His-Tag Chimeric antibody, Human Monoclonal

recombinant, expressed in HEK 293 cells, clone RMH01, purified immunoglobulin

Synonym(e):

Anti-His tag antibody

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Rekombinant

expressed in HEK 293 cells

Qualitätsniveau

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

RMH01, monoclonal
recombinant monoclonal

Form

buffered aqueous glycerol solution

Methode(n)

capture ELISA: 0.1-2 μg/mL
western blot: 0.1-1 μg/mL

Isotyp

IgG

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Hexahistidine tag (His-tag) consists of four to six histidines fused genetically. It is widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC).

Spezifität

This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Immunogen

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Leistungsmerkmale und Vorteile

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physikalische Form

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2


Analysenzertifikate (COA)

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins?evaluation of two anti-His-tag monoclonal antibodies
Muller KM, et al.
Analytical Biochemistry, 259(1), 54-61 (1998)
Jonathan F Lovell et al.
BMC medicine, 20(1), 462-462 (2022-11-30)
Numerous vaccine strategies are being advanced to control SARS-CoV-2, the cause of the COVID-19 pandemic. EuCorVac-19 (ECV19) is a recombinant protein nanoparticle vaccine that displays the SARS-CoV-2 receptor-binding domain (RBD) on immunogenic nanoliposomes. Initial study of a phase 2 randomized

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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