SAB4200878

Anti-Escherichia coli O111:B4 LPS, Mouse monoclonal

clone Ecco14, purified from hybridoma cell culture

• Synonym(e): E.coli bacteria
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Antikörperform: purified from hybridoma cell culture
• Qualitätsniveau: 200
• Klon: Ecco14
• Form: liquid
• Speziesreaktivität: E. coli
• Konzentration: ~1 mg/mL
• Isotyp: IgM
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. A typical LPS molecule has lipid A (a glucosamine-based phospholipid), a relatively short core oligosaccharide and a distal polysaccharide (O-antigen).

Spezifität

Monoclonal Anti-Escherichia coli O111:B4 LPS antibody specifically recognizes LPS of O111:B4 E. coli subtype and has no cross reactivity with Escherichia coli O26:B6 LPS, Escherichia coli eh100 LPS, P. gingivalis, P. mirabilis, S. typhimurium, S. enteritidis.

Anwendung

The antibody may be used in various immunochemical techniques including Immunoblotting and ELISA.

Biochem./physiol. Wirkung

LPSs contribute to the integrity of the outer membrane and protect the cell against the action of bile salts and lipophilic antibiotics. They stimulate host cells via TLR2 and TLR4 (toll like receptors). This stimulation results in the generation of various proinflammatory cytokines, such as TNFα (tumor necrosis factor), IL-1 (interleukin 1) and IL-6.1,2 Host cells stimulation later leads to hepatic secretion of acute phase proteins (APP) such as serum amyloid A (SAA) and haptoglobin (Hp).3,4 Acute phase proteins are known biomarkers for the diagnosis of several inflammatory conditions.5,6

Physikalische Form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Lagerung und Haltbarkeit

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Haftungsausschluss

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1