SAB4200716

Anti-Vimentin antibody, Mouse monoclonal

clone VIM-13.2, purified from hybridoma cell culture

• Synonym(e): Anti-F5H288
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: VIM-13.2, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: ~58 kDa
• Speziesreaktivität: mouse, bovine, canine, rat, monkey, human, chicken
• Erweiterte Validierung: independent; Learn more about Antibody Enhanced Validation
• Konzentration: ~1.0 mg/mL
• Methode(n): immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract; immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells; immunohistochemistry: suitable
• Isotyp: IgM
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: bovine ... Vim(280955) ; chicken ... Vim(420519) ; dog ... Vim(477991) ; human ... VIM(7431) ; mouse ... Vim(22352) ; rat ... Vim(81818) ; rhesus monkey ... Vim(705289)

Beschreibung

Allgemeine Beschreibung

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

Immunogen

human foreskin fibroblast extract

Anwendung

Anti-Vimentin antibody, Mouse monoclonal has been used in:

• western blotting
• indirect immunofluorescence
• immunohistochemistry

Biochem./physiol. Wirkung

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable