SAB4200094

Anti-PKM2 (isoform M1) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Synonym(e): Anti-CTHBP, Anti-OIP3 (OPA-interacting protein 3), Anti-PK3, Anti-PKM, Anti-Pyruvate Kinase, MUSCLE (isoform M1), Anti-TCB, Anti-THBP1 (thyroid hormone-binding protein, cytosolic)
• UNSPSC-Code: 12352203
• NACRES: NA.44

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~60 kDa
• Speziesreaktivität: human, rat, mouse
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~1.5 mg/mL
• Methode(n): immunocytochemistry: 5-10 μg/mL using HeLa cells; immunohistochemistry: 20-30 μg/mL using formalin-fixed paraffin embedded human colon; immunoprecipitation (IP): 2-4 μg using rat brain extract (S2 fraction); western blot: 1-2 μg/mL using mouse brain extract (S2 fraction)
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PKM2(5315)

Beschreibung

Allgemeine Beschreibung

Pyruvate Kinase (PK) is a key regulatory enzyme in glycolysis and has four known isoforms, namely, L, R, M1 and M2. The M2 isoform has been linked to cancer metastasis. PK isoform M1 is expressed during the development of the embryo and is the dominant isoform in cardiac, brain and skeletal muscle tissues.

Spezifität

Anti-PKM2 (isoform M1) antibody is specific for human, mouse and rat PKM2 (Isoform M1)/PKM1. In immunoblotting, detection of the PKM2 (Isoform M1) /PKM1 band is specifically inhibited by the immunizing peptide.

Anwendung

Anti-PKM2 (isoform M1) antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunohistochemistry
• immunocytochemistry

Biochem./physiol. Wirkung

Pyruvate kinase (PK) is a key enzyme in the glycolytic pathway. Knockdown of the M2 isoform in human cancer cell lines and its replacement by the M1 isoform has been shown to lead to reversal of the Warburg effect, and reduced ability to form tumors in mouse xenografts. Phosphorylation of the M2 isoform at Tyr¹⁰⁵ inhibits its activity and is common in human cancers, suggesting that Tyr¹⁰⁵ is a critical metabolic switch in cancer cells that promotes tumorigenesis.

Physikalische Form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable