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Key Documents

Weitere Dokumente

RAB0448

Sigma-Aldrich

Cell-Based ELISA for detecting phospho-STAT4 (pTyr693) in cultured cell lines

96 assays

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About This Item

UNSPSC-Code:
41116158

Verwendung

96 assays

Speziesreaktivität

mouse, human

Methode(n)

ELISA: suitable
capture ELISA: suitable

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

human ... STAT4(6775)
mouse ... STAT4(20849)

Verwandte Kategorien

Allgemeine Beschreibung

The Cell-Based Stat4 (pTyr693) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of Stat4 (pTyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines.

Immunogen

Stat4 (pTyr693) synthetic phosphopeptide

Anwendung

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB
  • RABBLOCK5x Blocking Solution (Item F)SDB
  • RABFIX1Fixing Solution (Item D)SDB
  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDB
  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDB
  • RABPLATE1Uncoated MicroplateSDB
  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDB
  • RABSTOP1Stop SolutionSDB
  • RABTMB1TMB Substrate Reagent (Item J)SDB
  • RABWASH120X Wash Buffer Concentrate ASDB
  • RABWASH220X Wash Buffer Concentrate BSDB
Alle anzeigen (10)

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

C M Bacon et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(16), 7307-7311 (1995-08-01)
Interleukin 12 (IL-12) is an important immunoregulatory cytokine whose receptor is a member of the hematopoietin receptor superfamily. We have recently demonstrated that stimulation of human T and natural killer cells with IL-12 induces tyrosine phosphorylation of the Janus family
K Yamamoto et al.
Molecular and cellular biology, 14(7), 4342-4349 (1994-07-01)
Interferon regulation of gene expression is dependent on the tyrosine phosphorylation and activation of the DNA-binding activity of two related proteins of 91 kDa (STAT1) and/or 113 kDa (STAT2). Recent studies have suggested that these proteins are substrates of Janus
Hua-Chen Chang et al.
The Journal of biological chemistry, 278(34), 32471-32477 (2003-06-14)
STAT4 (signal transducer and activator of transcription-4) mediates biological effects in response to interleukin-12 (IL-12). STAT4 has multiple domains that have distinct functions in signaling and gene activation. To characterize the role of the STAT4 N-terminal domain in mediating STAT4

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