P7306

Polyethylenglycol-/Dimethylsulfoxid-Lösung

Hybri-Max^™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

• Synonym(e): PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO
• MDL-Nummer: MFCD00081839
• UNSPSC-Code: 12161902
• PubChem Substanz-ID: 24898839
• NACRES: NA.75

Eigenschaften

• Qualität: Hybri-Max^™
• Sterilität: 0.2 μm filtered
• Produktlinie: BioReagent
• Form: solution
• Mol-Gew.: average mol wt 1,450
• Konzentration: 50 % (w/v)
• Methode(n): cell culture | hybridoma: suitable
• Verunreinigungen: endotoxin, tested
• Lagertemp.: 2-8°C
• SMILES String: C(CO)O
• InChI: 1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
• InChIKey: LYCAIKOWRPUZTN-UHFFFAOYSA-N

Beschreibung

Anwendung

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Verpackung

Packaged in sealed ampules under nitrogen.

Sonstige Hinweise

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Rekonstituierung

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Rechtliche Hinweise

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable