P3775

Anti-Protein A antibody produced in rabbit

fractionated antiserum, lyophilized powder

• Synonym(e): Anti-Protein A
• MDL-Nummer: MFCD00164723
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: fractionated antiserum
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: lyophilized powder
• Verpackung: vial of 2 mL lyophilized antiserum
• Methode(n): indirect ELISA: 1:100,000
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Protein A is a 42 kDa bacterial protein. It is isolated from the cell wall of Staphylococcus aureus.

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. To ensure specificity the fractionated antiserum is adsorbed using solid phase techniques, if necessary. Rabbit Anti-Protein A is lyophilized from 0.01 M phosphate buffered saline, pH 7.2, to which no preservatives have been added. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion versus anti-rabbit IgG results in a single arc of precipitation and versus anti-rabbit whole serum results in multiple arcs of precipitation.

Immunogen

Protein A from Staphylococcus aureus

Anwendung

Anti-Protein A antibody produced in rabbit has been used in immunoblotting.

Protein A may be used as in immunoblotting with a minimum dilution of 1:50,000.

Biochem./physiol. Wirkung

Protein A has an ability to bind Fc region of immunoglobulins from several species. It is used as a “universal tracer” and it facilitate the segregation of bound and free ligand. Protein A also acts as affinity matrices for antibody (IgG) purification.

Physikalische Form

Lyophilized from 0.01M phosphate buffered saline, pH 7.2

Rekonstituierung

Reconstitute with 2 ml deionized water.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)