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Merck

O4264

Sigma-Aldrich

Human Osteopontin

recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

Synonym(e):

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.32

product name

Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture

Biologische Quelle

human

Qualitätsniveau

Rekombinant

expressed in NSO cells

Sterilität

sterile

Assay

≥95% (SDS-PAGE)

Form

lyophilized powder

Mol-Gew.

60-65 kDa by SDS-PAGE (reducing)

Verpackung

pkg of 50 μg

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

UniProt-Hinterlegungsnummer

Versandbedingung

ambient

Lagertemp.

−20°C

Angaben zum Gen

human ... SPP1(6696)

Allgemeine Beschreibung

Secreted phosphoprotein 1 (SPP1) or osteopontin is a secreted cytokine. This phosphorylated acidic glycoprotein is expressed in various cells and tissues. It is synthesized by osteoblasts. The gene encoding SPP1 is localized on human chromosome 4q22.1.

Anwendung

Anti-Osteopontin antibody produced in rabbit has been used for ELISA.

Biochem./physiol. Wirkung

A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization. It is a marker of many cancers.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Hinweis zur Analyse

The biological activity is measured by its ability to mediate 293 cell adhesion.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Osteopontin Fragments with Intact Thrombin-Sensitive Site Circulate in Cervical Cancer Patients
Danny TM
PLoS ONE (2016)
Expanded comparative mapping between man
and rabbit and detection of a new conserved
segment between HSA22 and OCU4
C Chantry
Cytogenetic and genome research (2005)
Polycystic ovary syndrome is associated with increased osteopontin levels
European Journal of Endocrinology (2015)
Osteopontin That Is Elevated in the Airways during COPD Impairs the Antibacterial Activity of Common Innate Antibiotics
Anele Gela
PLoS ONE (2016)
Osteopontin-a possible anchor of osteoclasts to bone
FINN P. REINHOLT
Proceedings of the National Academy of Sciences of the USA (1990)

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