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MTOXCE2C9

Sigma-Aldrich

CypExpress 2C9 Cytochrome P450 human

Synonym(e):

CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, P450 2C9, P450IIC9

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About This Item

EG-Nummer:
UNSPSC-Code:
41106200
NACRES:
NA.77

Biologische Quelle

human

Rekombinant

expressed in Pichia pastoris

Form

dry powder

UniProt-Hinterlegungsnummer

Anwendung(en)

cell analysis

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... CYP2C9(1559)

Biochem./physiol. Wirkung

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Sulfaphenazole, fluconazole, fluvoxamine and fluoxetine are inhibitors, whereas rifampin is an inducer of the enzyme. The isozyme CYP2C9 is a major pathway for the invivo conversion of Diclofenac to 4-Hydroxydiclofenac via hydroxylation.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

Leistungsmerkmale und Vorteile

CypExpress 2C9 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 2C9 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

Einheitendefinition

A reaction mixture containing 500 μM diclofenac and 100 mg/mL of CypExpress 2C9 will produce 428 μM 4-hydroxydiclofenac in 2 hours at 30°C under the conditions described.

Physikalische Form

Light yellow, very fine powder

Rekonstituierung

Per Protocol/Variable

Rechtliche Hinweise

CypExpress is a trademark of Oxford Biomedical Research

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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