MTOX1303

Canine MDR1 Knockout, Human MDR1 Knockin MDCKII Cells

canine kidney (cocker spaniel)

• Synonym(e): MDCKII hMDR1 knockin cells
• UNSPSC-Code: 12352207
• NACRES: NA.81

Eigenschaften

• product name: Canine MDR1 Knockout, Human MDR1 Knockin MDCKII Cells,
• Biologische Quelle: canine kidney (cocker spaniel)
• Qualitätsniveau: 200
• Form: liquid
• Lagertemp.: −196°C
• Angaben zum Gen: human ... ABCB1(5243)

Beschreibung

Anwendung

See technical bulletin for detailed protocols

Leistungsmerkmale und Vorteile

MDCK II - Madin-Darby canine kidney- is a subclone derived from the heterogenous parent line - MDCK (ECACC catalogue no. 85011435) MDCK II cells, which predominate in later passages from MDCK, are reported to display electrical resistance of 100 ohm/cm2. This strain is thought to be derived from the distal tubule or collecting duct of the nephron. The cell line can be used as an experimental model to study the generation and maintenance of cell surface polarity in epithelial cells.

The canine MDR1 (cP-gp) efflux transporter gene has been effectively disrupted in both alleles. There is no expression of the cP-gp. Human MDR1 (P-gp) has been transfected into the MDCKII cell line. Validation studies have shown efflux of standard MDR1 substrates.

Qualität

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

Rechtliche Hinweise

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines LicenseMDCKII subclone was originally isolated by Daniel Louvard, Institut Curie, Paris France.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable