MAK213

Neutrophil Elastase Inhibitor Screening Kit

Sufficient for 100 Fluorometric tests

• Synonym(e): Neutrophil Elastase Inhibition Assay Kit
• EG-Nummer: 232-670-7
• UNSPSC-Code: 12352200
• NACRES: NA.25

Eigenschaften

• Nachweisverfahren: fluorometric
• Lagertemp.: −20°C
• Angaben zum Gen: human ... ELANE(1991) ; mouse ... ELANE(50701) ; rat ... ELANE(299606)

Beschreibung

Allgemeine Beschreibung

Neutrophil elastase (NE) is a serine protease that hydrolyzes proteins within the azurophilic granules of the neutrophils. Once secreted, it digests collagen-VI and elastin of the extracellular matrix. Neutrophil elastase is implicated in cystic fibrosis, bronchiectasis, chronic obstructive pulmonary disease, congenital neutropenia, and lung cancer.

Leistungsmerkmale und Vorteile

Compatible with high-throughput handling systems.

Eignung

Suitable for the screening of inhibitors of Neutrophil Elastase.

Prinzip

The Neutrophil Elastase Inhibitor Screening Kit is a rapid, sensitive and high throughput assay to screen and characterize potential inhibitors of NE. NE activity is measured by hydrolyzing the substrate to yield a fluorescent product (λ_ex = 400/λ_em = 505 nm) proportional to the enzymatic activity present.

Nur Kit-Komponenten

• Assay Buffer
• Substrate
• Neutrophil Elastase
• Inhibitor Control, 3 mM SPCK

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids