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MAK014

Sigma-Aldrich

Glucose-6-Phosphate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric tests

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

hematological disorder

Lagertemp.

−20°C

Allgemeine Beschreibung

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.

Anwendung

Glucose-6-phosphate assay kit has been used in:

  • glucose 6-phosphate (G6P) assay
  • quantification of glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P)
  • metabolite analysis

Eignung

Suitable for quantifying Glucose-6-Phosphate in a variety of samples.

Prinzip

G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazardCorrosion
GHS08,GHS05

Signalwort

Danger

H-Sätze

H314,H334

Gefahreneinstufungen

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Renal Disease in Type I Glycogen Storage Disease
Richard P L, et al.
Genetic Diseases of the Kidney (2009)
Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea
Shane M W, et al.
Plant Physiology, 112-112 (2013)
MiR-182 promotes glucose metabolism by upregulating hypoxia-inducible factor 1a in NSCLC cells
Mingliang W, et al.
Biochemical and Biophysical Research Communications, 504(2), 400-405 (2018)
Saixiang Feng et al.
Veterinary research, 51(1), 97-97 (2020-08-02)
Lipooligosaccharides (LOSs) are virulence determinants of Glaesserella parasuis, a pathogen of the respiratory tract of pigs. We previously reported that disruption of the galU or galE gene in G. parasuis results in increased sensitivity to porcine serum, indicating that the
Forkhead Box O6 (FoxO6) Depletion Attenuates Hepatic Gluconeogenesis and Protects against Fat-induced Glucose Disorder in Mice.
Virtu C N, et al.
The Journal of Biological Chemistry, 290, 15581-15594 (2015)
Alle zugehörigen Dokumente anzeigen

Artikel

Aerobic Glycolysis and the Warburg Effect

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

Protokolle

Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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