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M0535

Sigma-Aldrich

Mycoplasma-Bouillon

powder, suitable for microbiology

Synonym(e):

Mycoplasma media

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About This Item

UNSPSC-Code:
41106200
NACRES:
NA.74

Form

powder

Qualitätsniveau

200

Methode(n)

microbiological culture: suitable

Löslichkeit

H2O: 25.5 g/L (Autoclave at 121°C, 15 psi for 15 minutes. Store prepared medium at 0-5°C.)

Anwendung(en)

microbiology

Allgemeine Beschreibung

Mycoplasma Broth is a basal medium which, after enrichment with yeast extract, horse serum, and antibiotics, will support the growth of Mycoplasma sp. The components contained in this basal medium do not exhibit inhibitory or toxic effects when used to culture mycoplasma. A special mineral supplement has been added to improve the growth and colony characteristics of the organism.

Anwendung

Mycoplasma Broth has been used for isolation and cultivation of mycoplasma. It may also be used to grow Porphyromonas gingivalis under anerobic conditions.

Komponenten

Pulver enthält (g/L): 10.0 bakterielles Pepton, 10.0 LAB-LEMCO Pulver, 5.0 Natriumchlorid, 0.5 Mineral-Supplement.

Angaben zur Herstellung

Basal Medium:

1. Suspend 25.5 g of powdered medium in 1L of distilled water. Stir until the powder dissolves.

2. Adjust the pH of the solution to 7.8 ± 0.2.

3. Autoclave at 121 °C and 15 psi for 15 minutes to sterilize. Cool to 50 °C and add sterile supplements.

Lagerung und Haltbarkeit

Store dry powdered medium at room temperature and prepare medium at 2-8 °C.

Sonstige Hinweise

The product is intended for laboratory use only.

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Preisangaben

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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