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B1155

Sigma-Aldrich

bis-Benzimid H 33258

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Synonym(e):

2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazol), 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)-benzimidazol -trihydrochlorid, BBIH, HOE 33258, Hoechst 33258

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About This Item

Empirische Formel (Hill-System):
C25H24N6O · 3HCl
CAS-Nummer:
23491-45-4
Molekulargewicht:
533.88
Beilstein:
4088183
EG-Nummer:
245-690-6
MDL-Nummer:
MFCD00012679
UNSPSC-Code:
41106305
PubChem Substanz-ID:
24891565
NACRES:
NA.77

Produktlinie

BioReagent

Qualitätsniveau

200

Assay

≥98% (HPLC and TLC)

Form

powder

Methode(n)

cell culture | mammalian: suitable

Löslichkeit

H2O: 10 mg/mL
water and ethanol: 10 mg/mL

Eignung

passes application test for fluorescence

Lagertemp.

−20°C

SMILES String

Cl[H].Cl[H].Cl[H].[H]O[H].CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

InChIKey

OWRSPPSBNWJJAR-UHFFFAOYSA-N

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Anwendung

Zum Anfärben von DNA, Chromosomen und Zellkernen. Kann für Fluoreszenzmikroskopie oder Durchflusszytometrie verwendet werden.
Anregungsmaximum = 346 nm
Emissionsmaximum = 460 nm

Biochem./physiol. Wirkung

Membranpermeabeles, fluoreszierendes DNA-Färbemittel mit geringer Zytotoxizität, dass in A-T Regionen der DNA eingefügt wird.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Maria Elena Dell'Aquila et al.
Fertility and sterility, 91(1), 249-255 (2008-03-28)
To analyze the effects of GnRH agonists versus antagonists on mitochondrial distribution and activity in human mature oocytes. Randomized research experimental study. Academic basic research laboratory and hospital-based fertility center. Two hundred twenty-five supernumerary mature oocytes from 44 patients. Fluorescent
Luisa Valentini et al.
Animal reproduction science, 117(1-2), 166-177 (2009-04-18)
This study investigates the mitochondrial (mt) distribution in canine ovarian oocytes examined at recovery time, as related to the reproductive cycle stage, and in oviductal oocytes. Ovarian Germinal Vesicle (GV) stage oocytes were recovered from bitches in anestrous (A, n=2)
Luke O'Shaughnessy et al.
Methods in molecular biology (Clifton, N.J.), 681, 295-309 (2010-10-28)
Expression of recombinant proteins in the baculovirus/insect cell expression system is employed because it enables post-translational protein modification and high yields of recombinant protein. The system is capable of facilitating the functional expression of many proteins - either secreted or
Jared J Luxton et al.
Cell reports, 33(10), 108457-108457 (2020-11-27)
Telomere length dynamics and DNA damage responses were assessed before, during, and after one-year or shorter duration missions aboard the International Space Station (ISS) in a comparatively large cohort of astronauts (n = 11). Although generally healthy individuals, astronauts tended to
Luis Emiliano Peña-Altamira et al.
Neurochemistry international, 115, 37-49 (2017-10-25)
Purine nucleoside phosphorylase (PNP), a crucial enzyme in purine metabolism which converts ribonucleosides into purine bases, has mainly been found inside glial cells. Since we recently demonstrated that PNP is released from rat C6 glioma cells, we then wondered whether
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