L3892
Lektin aus Triticum vulgaris (Weizen)
peroxidase conjugate, lyophilized powder
Synonym(e):
Weizenkeim-Agglutinin
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Alle Fotos(2)
About This Item
UNSPSC-Code:
12352202
NACRES:
NA.32
Empfohlene Produkte
Biologische Quelle
Triticum vulgaris
Qualitätsniveau
Konjugat
peroxidase conjugate
Assay
≥85% protein basis (Warburg-Christian)
Form
lyophilized powder
Wirksamkeit
<40 μg/mL agglutination activity
peroxidase activity
50-200 units/mg protein
Zusammensetzung
Protein, ~90% modified Warburg-Christian
Lagertemp.
−20°C
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Anwendung
Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem./physiol. Wirkung
WGA ist nicht Blutgruppen-spezifisch, hat aber eine Affinität zu N-Acetyl-β-D-Glucosaminylresten und N-Acetyl-β-D-Glucosamin-Oligomeren. WGA enthält keine proteingebundenen Kohlenhydrate.
Verpackung
Packungsgrösse bezogen auf den Proteingehalt.
Einheitendefinition
1 U bildet 1.0 mg Purpurogallin aus Pyrogallol in 20 s bei pH 6.0 und 20 °C.
Physikalische Form
Enthält Citrat-Puffer
Angaben zur Herstellung
Konjugate werden aus affinitätsgereinigtem Lektin hergestellt.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Analysenzertifikate (COA)
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