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H8289

Sigma-Aldrich

Anti-dimethyl-Histone H1.4 (diMe-Lys26) in Kaninchen hergestellte Antikörper

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(e):

Anti-H1.4K26me2, Anti-H1K26me2

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Mol-Gew.

antigen ~35 kDa

Speziesreaktivität

human

Konzentration

~1.5 mg/mL

Methode(n)

western blot: 0.5-1 μg/mL using acid-extracted fraction of HL60 cells.

UniProt-Hinterlegungsnummer

P10412

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

dimethylation (Lys26)

Angaben zum Gen

human ... HIST1H1E(3008)

Spezifität

ChIP validated

Immunogen

dimethylated synthetic peptide corresponding to amino acids 22-23 (diMeLys26) of human histone H1.4.

Anwendung

Anti-dimethyl-Histone H1.4 (diMe-Lys26) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using acid-extracted fraction of HL60 cells.

Biochem./physiol. Wirkung

The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. In mammalian cells, four histone H1 variants (H1.2 to H1.5) are present in all somatic cells, and a fifth (H1.1) is restricted to thymus, testis, and spleen and possibly lymphocytic and neuronal cells. Histone H1.4 is di-methylated or acetylated at Lys26. Lys26 is located within the flexible N-terminal domain of H1.4, just preceding the globular domain.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

S Khochbin
Gene, 271(1), 1-12 (2001-06-19)
Genes encoding linker histone variants have evolved to link their expression to signals controlling the proliferative capacities of cells, i.e. cycling and growth-arrested cells express distinct and specific H1 subtypes. In metazoan, these variants show a tripartite structure, with considerably
Papita Mandal et al.
The FEBS journal, 281(23), 5292-5308 (2014-09-30)
Glutamate dehydrogenase has been recently identified as a tissue-specific histone H3-specific clipping enzyme. We have previously shown that it cleaves free as well as chromatin-bound histone H3. However, the physiological significance of this enzyme is still not clear. The present
J C Rice et al.
Current opinion in cell biology, 13(3), 263-273 (2001-05-10)
Post-translational addition of methyl groups to the amino-terminal tails of histone proteins was discovered more than three decades ago. Only now, however, is the biological significance of lysine and arginine methylation of histone tails being elucidated. Recent findings indicate that

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