H8289

Anti-dimethyl-Histone H1.4 (diMe-Lys²⁶) in Kaninchen hergestellte Antikörper

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-H1.4K26me2, Anti-H1K26me2
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~35 kDa
• Speziesreaktivität: human
• Konzentration: ~1.5 mg/mL
• Methode(n): western blot: 0.5-1 μg/mL using acid-extracted fraction of HL60 cells.
• UniProt-Hinterlegungsnummer: P10412
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: dimethylation (Lys26)
• Angaben zum Gen: human ... HIST1H1E(3008)

Beschreibung

Spezifität

ChIP validated

Immunogen

dimethylated synthetic peptide corresponding to amino acids 22-23 (diMeLys²⁶) of human histone H1.4.

Anwendung

Anti-dimethyl-Histone H1.4 (diMe-Lys26) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using acid-extracted fraction of HL60 cells.

Biochem./physiol. Wirkung

The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. In mammalian cells, four histone H1 variants (H1.2 to H1.5) are present in all somatic cells, and a fifth (H1.1) is restricted to thymus, testis, and spleen and possibly lymphocytic and neuronal cells. Histone H1.4 is di-methylated or acetylated at Lys26. Lys26 is located within the flexible N-terminal domain of H1.4, just preceding the globular domain.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)