H4538

Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal

clone HDAC5-35, purified from hybridoma cell culture

• Synonym(e): Anti-HD5
• MDL-Nummer: MFCD04040361
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified from hybridoma cell culture
• Antikörper-Produkttyp: primary antibodies
• Klon: HDAC5-35, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~124 kDa
• Speziesreaktivität: rat, mouse, human
• Konzentration: ~2 mg/mL
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 4-8 μg/mL using total cell extracts of NIH3T3 fibroblast cell or SW480 cell extract
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: Q9UQL6
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HDAC5(10014) ; mouse ... Hdac5(15184) ; rat ... Hdac5(84580)

Beschreibung

Allgemeine Beschreibung

Monoclonal Anti-Histone Deacetylase 5 (HDAC5) (mouse IgG1 isotype) is derived from the HDAC5-35 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide. Mammalian HDACs can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and HDAC10. Histone deacetylase 5 (HDAC5) is a histone deacetylase, located on human chromosome 17q21.

Anwendung

Anti-Histone Deacetylase 5 (HDAC5) antibody, Mouse monoclonal has been used in:

• western blotting
• immunostaining
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunoprecipitation

Biochem./physiol. Wirkung

Histone deacetylase 5 (HDAC5) promotes progression of the cell cycle, cell proliferation and apoptosis. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (Mef2) protein, thus repressing expression of MEF2 downstream genes. Overexpression of HDAC5 in different cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. Reduced expression of the protein is observed in colon cancer and acute myeloid leukemia.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg