H2126

Hyaluronidase aus Schaftestes

Type II, lyophilized powder, ≥300 units/mg solid

• Synonym(e): Hyaluronat-4-glycanhydrolase, Hyaluronoglucosaminidase
• CAS-Nummer: 37326-33-3
• EC-Nummer: 3.2.1.35 (BRENDA, IUBMB)
• EG-Nummer: 253-464-3
• MDL-Nummer: MFCD00131351
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Konjugat: conjugate (Glucosaminoglycan)
• Typ: Type II
• Form: lyophilized powder
• Spezifische Aktivität: ≥300 units/mg solid
• Mol-Gew.: 55 kDa
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Anwendung

Hyaluronidase from sheep testes has been used:

• in flow cytometry
• in the isolation of putative spermatogonial stem cells (SSCs)
• to study the effects of magnetic stimulation in magnetic hydrogel properties
• in immunohistochemistry to visualize collagen type II distribution
• to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
• for the degeneration of hydrogels

Biochem./physiol. Wirkung

Diese Enzyme spalten randomisiert β-N-acetylhexosamin-[1−4]-glycosidische Bindungen in Hyaluronsäure, Chondroitin und Chondroitin-Sulfaten.

Einheitendefinition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Physikalische Form

Lyophilized powder containing lactose

Sonstige Hinweise

Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)