GERPN3682
CDP-Star®
Cytiva RPN3682
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About This Item
UNSPSC-Code:
12352204
NACRES:
NA.83
Empfohlene Produkte
Form
liquid
Verpackung
pkg of 1 kit
Hersteller/Markenname
Cytiva RPN3682
Versandbedingung
wet ice
Lagertemp.
2-8°C
Allgemeine Beschreibung
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star detection reagent allows for chemiluminescent detection of Alkaline Phosphatase labelled nucleic acids.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Sufficient reagent for 2500cm² of membrane. Can be used in conjunction with AlkPhos Direct Labeling Module (RPN3680), Amersham AlkPhos Direct Hybridization Buffer (RPN3688) and Hybond/ Nytran membranes.
Each system includes the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500 cm2 or 5000 cm2 membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star or ECF™ detection reagents.
Anwendung
CDP-Star® has been used to label and quantify genomic DNA by Southern blotting.
Leistungsmerkmale und Vorteile
- Probes are labelled during a 30 minute reaction, resulting in probes that can be used without purification.
- Hybridization stringency can be controlled by temperature as well as salt concentration. Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
- Detection is rapid, taking only 30 minutes from completion of hybridization to application of detection reagent.
Lagerung und Haltbarkeit
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Hinweis zur Analyse
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Rechtliche Hinweise
CDP-Star is a registered trademark of Tropix, Inc.
ECF is a trademark of Cytiva
Signalwort
Danger
H-Sätze
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
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Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
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