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GE28-9673-90

His Mag Sepharose™ Ni

Cytiva 28-9673-90, pack of 5 × 1 mL

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

ligand

IMAC immobilized with Ni

Verpackung

pack of 5 × 1 mL

Hersteller/Markenname

Cytiva 28-9673-90

Parameter

Room temperature temp. range

Matrix

paramagnetic, spherical, highly cross-linked agarose particles

Kapazität

50 mg binding capacity(histidine-tagged protein/ml medium)

Allgemeine Beschreibung

His Mag Sepharose™ Ni is magnetic beads designed to simplify small-scale purification and expression screening of histidine-tagged proteins.

Anwendung

His Mag Sepharose™ Ni products are magnetic beads designed for rapid, small-scale purification and screening of histidine-tagged Proteins from different sources. Mag Sepharose™ format has excellent properties for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices while the visibility of the beads ensures reliable collection of the bound histidine-tagged Proteins in the purification procedure.

The product is based on IMAC Sepharose™ medium with magnetite incorporated and nickel immobilized.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel.

Leistungsmerkmale und Vorteile

  • High capacity magnetic beads for small-scale purification and screening of histidine-tagged Proteins
  • Easy to use visible and dense magnetic beads
  • High purity and yield
  • Easy parallel screening with high repeatability
  • Scalable-simple capture of target Protein from small μL to high mL sample volumes

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Environment
GHS09

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

EU REACH Annex XVII (Restriction List)

CAS No.

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protokolle

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and subsequently charged with Ni2+ ions.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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