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GE28-9365-50

GSTPrep™ Fast Flow 16/10

Cytiva 28-9365-50

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About This Item

UNSPSC-Code:
13111000
NACRES:
NA.56

ligand

glutathione and 10-carbon linker arm

Verpackung

pkg of 20 mL

Hersteller/Markenname

Cytiva 28-9365-50

Parameter

<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

Bettgröße

16 mm × 100 mm

Bettvolumen

20 mL

Säule ID

16 mm

Matrix

highly cross-linked 4% agarose

Durchschnittlicher Durchmesser

90 μm

cleaning

3-12

working range

3-12

Kapazität

>10 mg/mL binding capacity

Allgemeine Beschreibung

GSTPrep™ FF 16/10 is prepacked, ready-to-use column containing Glutathione Sepharose™ 4 Fast FLow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Anwendung

Glutathione Sepharose™ 4 Fast FLow is for scale-up purification of recombinant GST-tagged Proteins, other glutathione S-transferases, and glutathione-dependent Proteins. Mild elution conditions preserve Protein antigenicity and function.

Leistungsmerkmale und Vorteile

  • Fast and easy one-step, scale-up purification of GST-tagged Proteins.
  • Binds approx. 200 mg recombinant GST/column
  • Mild elution conditions preserving Protein antigenicity and function.
  • Simple operation with a pump or chromatography system, such as ÄKTA® design.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

GSTPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

Alle anzeigen

Protokolle

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Performing a Separation with Cytiva Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.

Purification Using GSTrap™ HP, GSTrap™ FF, and GSTrap™ 4B Columns

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap columns packed with Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, or Glutathione Sepharose® 4B chromatography media.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

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