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GE28-9365-44

HiPrep Sp Fast Flow 16/10

Cytiva 28-9365-44

Synonym(e):

Fast Flow Column, Fast Flow SP Column, HiPrep SP Column

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About This Item

UNSPSC-Code:
47101511
NACRES:
NA.56

ligand

sulphopropyl

Beschreibung

Ion Exchanger Type (value)

Verpackung

pkg of 1 ea

Hersteller/Markenname

Cytiva 28-9365-44

Parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

Bettgröße

16 mm × 100 mm

Bettvolumen

20 mL

Säule ID

16 mm

Matrix

6% cross-linked agarose

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning

3-14

Arbeitsbereich

4-13

Eignung

suitable for bioprocess medium

Allgemeine Beschreibung

HiPrep SP FF is a strong cation exchanger prepacked with SP Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

HiPrep SP FF are prepacked, ready to use columns for ion exchange chromatography. They provide fast, preparative separations of proteins and other biomolecules.

Leistungsmerkmale und Vorteile

  • HiPrep columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • Good flow rates and high capacity.
  • The industry standard for ion exchange chromatography.
  • Compatible with single-pump configurations and chromatography systems such as AKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.2 M sodium acetate in 20% ethanol)
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Sonstige Hinweise

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Rechtliche Hinweise

HiPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva

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Signalwort

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H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


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Analysenzertifikate (COA)

Lot/Batch Number

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Artikel

This page shows how to perform a purification of His-tagged membrane proteins.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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Protokolle

This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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